• Species Reactivity

  Mouse

• Specificity

  Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.

• Source

  Monoclonal Rat IgG2a Clone # 223322

• Immunogen

  BaF/3 mouse pro-B cell line transfected with mouse LYVE-1    
  Ala24-Thr234    
  Accession # Q8BHC0

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  bEnd.3 mouse endothelioma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

• References:

1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.

2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.

3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.

4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.

5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.

6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.

7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.

8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.

9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.

11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

• Long Name:

  Lymphatic Vessel Endothelial Hyaluronan Receptor 1

• Entrez Gene IDs:

  10894 (Human); 114332 (Mouse); 293186 (Rat)

• Alternate Names:

  cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1