• Species Reactivity

  Human

• Specificity

  Detects human BMP‑5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP-4, rhBMP-6, rhBMP-7, and rhBMP-8 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human BMP‑5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Human BMP‑5 (Catalog # )

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Paraffin-embedded sections of human placenta and osteosarcoma

• 
  

• Neutralization

  Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. Erlacher, L.     et al. (1998) J. Bone Miner. Res.     13:383. The Neutralization Dose (ND    ₅₀) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑5 and 50 µg/mL L-ascorbic acid.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-5

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence identity with mouse and rat BMP-5. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (3). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the   short ear phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).

• References:

1. Chen, D. et al. (2004) Growth Factors 22:233.

2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.

3. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.

4. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.

5. King, J.A. et al. (1994) Dev. Biol. 166:112.

6. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.

7. Pierre, A. et al. (2005) Biol. Reprod. 73:1102.

8. Beck, H.N. et al. (2001) BMC Neurosci. 2:12.

9. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.

10. Jin, Y. et al. (2001) Oral Oncol. 37:225.

11. Ro, T.B. et al. (2004) Oncogene 23:3024.

• Long Name:

  Bone Morphogenetic Protein 5

• Entrez Gene IDs:

  653 (Human); 12160 (Mouse)

• Alternate Names:

  BMP5; BMP-5; bone morphogenetic protein 5; MGC34244