• Species Reactivity

  Human

• Specificity

  Detects human Importin alpha 2/KPNA2 in Western blots.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Importin alpha 2/KPNA2    
  Met1-Asp132    
  Accession # P52292

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Immunocytochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 2/KPNA2

KPNA2 (Karyopherin subunit alpha 2; also SRP1 alpha, importin subunit alpha 2 and RAG1) is a 58-60 kDa member of the importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, KPNA2 first binds to NBS1, and then to importin beta 1, which mediates movement through the nuclear pore. Human KPNA2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/importin  beta domain (aa 2-60), ten Armadillo repeats that bind "cargo" (aa 71-496) and three intervening NLSs. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human KPNA2 shares 94% aa identity with mouse KPNA2.

• Long Name:

  Karyopherin alpha 2

• Entrez Gene IDs:

  3838 (Human); 16647 (Mouse); 85245 (Rat)

• Alternate Names:

  Importin alpha 1; Importin alpha 2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); Karyopherin subunit alpha-2; KPNA2; Pendulin; QIP2; QIP2importin alpha 2; RAG cohort 1; RAG cohort protein 1; RCH1; RCH1importin subunit alpha-2; SRP1; SRP1alpha; SRP1-alpha