• Species Reactivity

  Human

• Specificity

  Detects human Gastrokine 1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse Gastrokine 1 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Gastrokine 1    
  Asn35-Asn199    
  Accession # Q9NS71

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gastrokine 1

Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54‑150) that contains a mitogenic sequence (aa 97‑117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21‑185 (aa 35‑199 of SwissProt Q9NS71), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.

• Long Name:

  Take out Column D name

• Entrez Gene IDs:

  56287 (Human); 66283 (Mouse); 297418 (Rat)

• Alternate Names:

  18 kDa antrum mucosa protein; AMP18; AMP-18; AMP18BRICHOS domain containing 1; BRICD1; CA11; CA11MGC70354; FOV; Foveolin; Gastrokine 1; gastrokine-1; GKN1; Protein CA11