• Species Reactivity

  Human

• Specificity

  Detects human LPAR2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LPAR1 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human LPAR2    
  Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279    
  Accession # Q9HBW0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR2/LPA2/EDG-4

LPAR2 (Lysophosphatidic acid receptor 2; also LPA₂ and EDG4) is a 39 kDa member of the GPCR1 family of proteins.  It has limited expression, and is found on mammalian macrophages, fibroblasts and cardiac myocytes.  LPAR2 binds to, and is activated by LPA, a molecule principally derived from activated platelets.  Although LPAR2 appears to prefer myristoyl and palmitoyl LPA, multiple forms of LPA are recognized by the receptor.  LPAR2 reportedly mediates chemotaxis, arachidonic acid release, and cell rounding.  Human LPAR2 is a 351 amino acid (aa) 7–transmembrane (TM) protein (SwissProt # Q9HBW0).  It contains a 33 aa extracellular N-terminus (aa #1-33), seven TM segments (aa #34-297) and a 54 aa C-terminal cytoplasmic tail.  There is one splice variant that is associated with tumor cells, and which originally was given the designation EDG4 (endothelium differentiation gene 4).  This shows a 35 aa substitution for aa #348-351.  Over aa #1-33, 91-103, 168-188 and 264-279 collectively, human LPAR2 shares 75% aa identity with mouse LPAR2.  

• Long Name:

  Lysophosphatidic Acid Receptor 2

• Entrez Gene IDs:

  9170 (Human); 53978 (Mouse); 498609 (Rat)

• Alternate Names:

  EDG4; EDG-4; EDG4FLJ93869; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor4,LPA receptor EDG4; G protein-coupled receptor; LPA2; LPA-2; LPA2LPA receptor 2; LPAR2; lysophosphatidic acid receptor 2; lysophosphatidic acid receptor EDG4; Lysophosphatidic acid receptor Edg-4