• Species Reactivity

  Human

• Specificity

  Detects human Spk2 in direct ELISAs and Western blots.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Skp2    
  Leu130-Pro350    
  Accession # Q13309

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Skp2

Skp2 (S-phase kinase-associated protein 2; also F-box/LRR protein 1) is a 45-49 kDa member of the F-box protein family. It is known to be part of the SCF (Skp1/Cul1/F-box protein) complex. The SCF complex functions as an E3 ubiquitin-protein ligase that mediates the transfer of ubiquitin from an E2 enzyme to a target substrate. The specificity of the target is determined by Skp2. Molecules targeted for degradation include cell-cycle regulatory proteins that are involved in the G1-S phase transition. Human Skp2 is 424 amino acids (aa) in length. It contains one F-box (aa 94-140) followed by nine consecutive Leu-rich repeats (aa 142-369). There are two potential splice forms that contain substitutions for the same aa sequence. One contains a 4 aa substitution, while a second contains a 56 aa substitution for aa 354-424. Over aa 130-350, human Skp2 shares 84% aa identity with mouse Skp2.

• Long Name:

  S Phase Kinase-associated Protein 2

• Entrez Gene IDs:

  6502 (Human); 27401 (Mouse); 294790 (Rat)

• Alternate Names:

  CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FBXL1MGC1366; FLB1; p45skp2; Skp2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2