• Species Reactivity

  Human

• Specificity

  Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CT-1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human CLC    
  Leu28-Phe225    
  Accession # Q9UBD9

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Human CLC (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLC

Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8).  Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

• References:

1. Murakami, M. et al. (2004) Growth Factors 22:75.

2. Ernst, M. & B.J. Jenkins (2004) Trends Genet. 20:23.

3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.

4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.

5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.

6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.

7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.

8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.

9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854.

10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.

11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.

12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.

• Long Name:

  Cardiotrophin-like Cytokine

• Entrez Gene IDs:

  23529 (Human); 56708 (Mouse); 365395 (Rat)

• Alternate Names:

  B-cell stimulating factor 3; B-cell stimulatory factor 3; BSF-3; BSF3BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLC; CLCF1; CLCNNT-1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT-1; NNT1B-cell-stimulating factor 3; Novel neurotrophin-1; NR6