• Species Reactivity

  Mouse

• Specificity

  Detects mouse GDF-1 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant mouse (rm) GDF-1 and rmGDF-3 Propeptide is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse GDF-1 Propeptide    
  Pro25-Leu235    
  Accession # P20863

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse GDF‑1 Propeptide

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-1

Mouse GDF-1 Propeptide is a 26 kDa, secreted monomeric glycopeptide. It is synthesized as part of a 357 amino acid (aa) preprocursor that contains a 23 aa signal sequence, a 214 aa Propeptide and a 120 aa mature molecule. Following secretion, the proregion is presumably proteolytically cleaved from the mature molecule at an Arg-Arg dibasic residue motif. The amino acid sequence of mouse GDF-1 Propeptide is 65% and 78% identical to that of human and rat GDF-1 Propeptide, respectively.

• Long Name:

  Growth Differentiation Factor 1

• Entrez Gene IDs:

  2657 (Human); 14559 (Mouse)

• Alternate Names:

  DORV; DTGA3; embryonic growth/differentiation factor 1; GDF1; GDF-1; growth differentiation factor 1