• Species Reactivity

  Mouse

• Specificity

  Detects mouse NPRA/NPR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) NPR1 is observed and 5% cross-reactivity with rhNPR2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse NPRA/NPR1    
  Ser29-Glu469    
  Accession # P18293

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse NPRA/NPR1

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRA/NPR1

Mouse Natriuretic Peptide Receptor-1 (NPR1), also known as NPRA, ANP-R1 or guanlyl cyclase-A, is a 130 kDa member of the guanylyl cyclase family of proteins. NPR1 is a 1029 amino acid type I transmembrane glycoprotein that contains a 441 amino acid extracellular domain and a 567 amino acid cytoplasmic region. The cytoplasmic region shows a tyrosine kinase and guanylyl cyclase catalytic domain. NPR1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type n.p.) ANP binding induces bronchodilation and a reduction in blood pressure. Over the extracellular domain, mouse NPR1 is 97% and 86% identical to rat and human NPR1, respectively.

• Long Name:

  Natriuretic Peptide Receptor A

• Entrez Gene IDs:

  4881 (Human); 18160 (Mouse); 24603 (Rat)

• Alternate Names:

  ANPa; ANP-A; ANPRA; ANPR-A; ANPRAEC 4.6.1.2; atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; Guanylate cyclase A; GUC2A; GUCY2A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptidereceptor A); NPR1; NPRA; NPR-A; Pndr