• Species Reactivity

  Mouse

• Specificity

  Detects mouse THRSP in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse THRSP    
  Gly47-Leu150    
  Accession # Q62264

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THRSP

THRSP (Thyroid hormone inducible hepatic protein; also S14/SPOT 14) is an 18-20 kDa acidic nuclear protein that belongs to the SPOT14 family of molecules. It is expressed by lactating mammary epithelium, adipocytes and hepatocytes, and appears to promote the synthesis of long-chain fatty acids. Its synthesis is induced by thyroid hormone, insulin and carbohydrates. Mouse THRSP is 150 amino acids (aa) in length. The residues between aa 10-66 contain a Leu-zipper domain that mediates noncovalent homodimerization plus heterodimerization with MIG12. Other proteins known to bind to THRSP include the thyroid hormone receptor and Zac51. Heterointeractions are posited to positively influence p53-dependent gene activation. Two potential isoforms are reported. One contains a seven aa extension at the C-terminus, while another shows a deletion of aa 31-64. Over aa 47-150, mouse THRSP shares 78% and 94% aa identity with human and rat THRSP, respectively.

• Long Name:

  Thyroid Hormone Responsive [SPOT14 Homolog] Protein

• Entrez Gene IDs:

  7069 (Human); 21835 (Mouse); 25357 (Rat)

• Alternate Names:

  lipogenic protein 1; Lpgp; LPGP1; S14; S14MGC21659; Spot 14 protein; SPOT14; SPOT14SPOT14 homolog; THRSP; thyroid hormone responsive (SPOT14 homolog, rat); thyroid hormone responsive SPOT14 (rat) homolog; thyroid hormone responsive SPOT14; thyroid hormone responsive; thyroid hormone-inducible hepatic protein