• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human CD81 LEL Fc Chimera (Catalog # ) is coated onto a microplate at 2 μg/mL, Recombinant Viral HCV E2 binds with an ED    ₅₀ of 0.2-1.2 µg/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Glu384-Glu661, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Glu384

• Predicted Molecular Mass

  31 kDa

• SDS-PAGE

  63-74 kDa, reducing conditions

Data Images

Background: HCV E2

Glycoprotein E2 is an approximately 60 kDa viral envelope protein encoded by the hepatitis C virus (HCV) genome (1-3). HCV infection is closely associated with the development of hepatocellular carcinoma and cirrhosis of the liver. E2 is a transmembrane molecule expressed as a noncovalent dimer with the E1 glycoprotein (4). It binds to CD81 and CD36/SR-BI on hepatocytes, and glycosylation of E2 is important for this interaction (4-8). The virus also requires target cell expression of the tight junction proteins Claudin-1 and Occludin for infectivity (7, 9, 10). CD81 and Occludin are responsible for the human host cell specificity of HCV (10). E2 binding to CD81 on NK cells inhibits cytokine production and cytolytic activity (11, 12). In contrast, E2 binding to CD81 on T cells provides a costimulatory signal (11, 12). On plasmacytoid dendritic cells, E2 binds to CD81 as well as BDCA-2 and DCIR, resulting in inhibition of IFN-alpha production and cell maturation (13, 14).

• References:

1. Khan, A.G. et al. (2015) Curr. Opin. Virol. 12:53.

2. Zona, L. et al. (2014) Viruses 6:875.

3. Grakoui, A. et al. (1993) J. Virol. 67:1385.

4. Op De Beeck, A. et al. (2004) J. Virol. 78:2994.

5. Pileri, P. et al. (1998) Science 282:938.

6. Scarselli, E. et al. (2002) EMBO J. 21:5017.

7. Harris, H.J. et al. (2010) J. Biol. Chem. 285:21092.

8. Falkowska, E. et al. (2007) J. Virol. 81:8072.

9. Evans, M.J. et al. (2007) Nature 446:801.

10. Ploss, A. et al. (2009) Nature 457:882.

11. Crotta, S. et al. (2002) J. Exp. Med. 195:35.

12. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.

13. Tu, Z. et al. (2013) Cell. Immunol. 284:98.

14. Florentin, J. et al. (2012) Blood 120:4544.

• Long Name:

  Hepatitis C Virus 1b Envelope Glycoprotein E2

• Entrez Gene IDs:

  951475 (Viral)

• Alternate Names:

  HCV E2; HCV-E2; hcvGP-E2; Hep C E2