• Species Reactivity

  Human

• Specificity

  Detects human Phospho-Axl (Y779) in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Mouse IgG1 Clone # 713610

• Immunogen

  Phosphopeptide containing the human Axl Y779 site

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Pervanadate-treated A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with Triton® X-100

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Phosphorylation of Tyrosine 779 provides a docking site for p85 subunits of PI 3-Kinase (4).

• References:

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.

2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.

3. Holland, S. et al. (2005) Canc. Res. 65:9294.

4. Weinger, J.G. et al. (2008) J. Neurochem. 106:134.
   

• Long Name:

  Axl Receptor Tyrosine Kinase

• Entrez Gene IDs:

  558 (Human); 26362 (Mouse)

• Alternate Names:

  Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL