• Species Reactivity

  Human

• Specificity

  Detects human MMP‑15/MT2‑MMP in direct ELISAs and Western blots. Does not detect the catalytic domain of     E. coli-expressed recombinant human MMP-15 (aa 132‑304).

• Source

  Monoclonal Mouse IgG1 Clone # 130522

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human MMP-15    
  aa 132-625

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  SW480 human colorectal adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-15/MT2-MMP

MMP-15, also known as membrane-type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.

• Long Name:

  Matrix Metalloproteinase 15/Membrane Type 2 MMP

• Entrez Gene IDs:

  4324 (Human)

• Alternate Names:

  EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15 (membrane-inserted); Membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase; MMP15; MMP-15; MT2-MMP; MT2-MMPMT2MMP; MTMMP2MT-MMP 2; SMCP-2matrix metalloproteinase-15