• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 2 x 10    ⁴ cells/well are added to Recombinant Human MAGP-2/MFAP5 coated plates (10 µg/mL with 100 µL/well), approximately 50-75% will adhere after one hour at 37 °C.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived Ile22-Leu173 with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ile22 & Ser154

• Structure / Form

  Monomer

• Predicted Molecular Mass

  18.1 kDa

• SDS-PAGE

  25-35 kDa, reducing conditiions

Background: MAGP-2/MFAP5

MAGP-2 (Microfibril-associated glycoprotein 2), also called MFAP5 (microfibril associated protein 5) is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins (1). Human MAGP-2 cDNA encodes 173 amino acids (aa) including a 21 aa signal sequence, an RDG motif (aa 30 ‑ 32; absent in MAGP-1) and a central cysteine‑rich binding domain (aa 84 ‑ 140) (1 ‑ 3). One potential splice variant shows a 10 aa deletion between aa 73 ‑ 82 that includes the only potential glycosylation site (4). Mature human MAGP-2 shares 80%, 82%, 82%, 84% and 85% aa identity with mouse, rat, bovine, porcine and canine MAGP-2, respectively. Both MAGP-1 and MAGP-2 bind and covalently crosslink fibrillins 1 and 2  through the binding domain, but MAGP-2 has more limited distribution and binds a more limited set of extracellular matrix proteins (1 ‑ 3). However, the MAGP-2 RGD motif binds to the integrin alpha v beta 3, allowing cell attachment to microfibrils (5). MAGP‑2 is thought to facilitate microfibril assembly and induce elastin fiber formation (1 ‑ 3, 6). Through its binding to EGF repeats, MAGP-2 mediates the metalloproteinase-dependent release of Jagged1 and the metalloproteinase-independent release of Notch extracellular domains (7, 8). In endothelial cells, MAGP-2 promotes angiogenic sprouting through inhibiting Notch signaling, binding integrin  alpha v beta 3 (which then enhances VEGF signaling), and/or enhancing endothelial cell response to FGF basic and EGF (9, 10). In ovarian cancer, MAGP-2 expression is associated with cancer cell survival, increased microvessel density, and poor prognosis (11). Skin MAGP-2 expression is increased in human scleroderma and the tight skin (TSK) mouse; MAGP‑2 may contribute to abnormal collagen accumulation in these conditions by increasing the half-life of type I collagen (12, 13). Fibrillin-1 mutations within the region that binds MAGP-2 can be found in Marfan syndrome (6).

• References:

1. Gibson, M.A. et al. (1996) J. Biol. Chem. 271:1096.

2. Hanssen, E. et al. (2004) J. Biol. Chem. 279:29185.

3. Segade, F. et al. (2002) J. Biol. Chem. 277:11050.

4. Genbank protein Accession # AAQ18021.

5. Gibson, M.A. et al. (1999) J. Biol. Chem. 274:13060.

6. Lemaire, R. et al. (2007) J. Biol. Chem. 282:800.

7. Nehring, L.C. et al. (2005) J. Biol. Chem. 280:20349.

8. Miyamoto, A. et al. (2006) J. Biol. Chem. 281:10089.

9. Albig, A.R. et al. (2008) Microvasc. Res. 76:7.

10. Albig, A.R. et al. (2007) Angiogenesis 10:197.

11. Mok, S.C. et al. (2009) Cancer Cell 16:521.

12. Lemaire, R. et al. (2005) Arthritis Rheumatol. 52:1812.

13. Ito, S. et al. (2006) Am.J. Respir. Cell Mol. Biol. 34:688.

• Long Name:

  Microfibril-associated Glycoprotein 2

• Entrez Gene IDs:

  8076 (Human); 50530 (Mouse); 362429 (Rat)

• Alternate Names:

  MAGP2; MAGP-2; MAGP2MFAP-5; MFAP5; Microfibril-associated glycoprotein 2; microfibrillar associated protein 5; microfibrillar-associated protein 5; MP25; MP25microfibril-associated glycoprotein-2