• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10    ⁴ cells/well are added to Recombinant Human ASAM coated plates (30 µg/mL, 100 µL/well), approximately 25%‑50% will adhere after one hour at 37 °C.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Chinese Hamster Ovary cell line, CHO-derived Gly18-Met233, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Gly18

• Predicted Molecular Mass

  25.2 kDa

• SDS-PAGE

  33-37 kDa, reducing conditions

Background: ASAM

Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and coxsackie- and adenovirus receptor-like membrane protein (CLMP), is a type I transmembrane protein and member of the CTX family within the immunoglobulin superfamily. Mature human ASAM consists of a 217 amino acid (aa) extracellular region, a 21 aa transmembrane region, and a 117 aa cytoplasmic tail. The extracellular region contains one V-type and one C-type immunoglobulin domain connected by a J segment (1, 2). Within the ECD, human ASAM shares 97% aa sequence identity with mouse and rat ASAM. ASAM is widely expressed as 44 kDa and 48 kDa species in epithelial tissues where it localizes to tight junctions (1, 3). It mediates cell‑cell adhesion and contributes to trans-epithelial resistance (1). It is down‑regulated in Sertoli cells by TNF-alpha which also inhibits the expression of several other tight junction proteins in seminiferous tubules (4, 5). ASAM is additionally expressed by mature adipocytes and is up‑regulated in white adipose tissue under conditions of obesity (2).

• References:

1. Raschperger et al. (2004) J. Biol. Chem. 279:796.

2. Eguchi, J. et al. (2005) Biochem. J. 387:343.

3. Sze, K.-L. et al. (2008) J. Cell Physiol 214:334.

4. Sze, K.-L. et al. (2008) Biochem. J. 410:575.

5. Li, M.W. et al. (2006) J. Endocrinol. 190:313.

• Long Name:

  Adipocyte-specific Adhesion Molecule

• Entrez Gene IDs:

  79827 (Human); 71566 (Mouse); 286939 (Rat)

• Alternate Names:

  ACAM; ACAMCoxsackie- and adenovirus receptor-like membrane protein; Adipocyte adhesion molecule; adipocyte-specific adhesion molecule; ASAM; ASAMCAR-like membrane protein; Asp5; CLMP; CXADR-like membrane protein; FLJ22415