详细说明
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
Source
Mouse myeloma cell line, NS0-derived Gln26-Met271, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained: Gln26 predicted
Predicted Molecular Mass
27 kDa
SDS-PAGE
36 kDa, reducing conditions
5025-CL |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CL-K1/COLEC11
Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain an N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the blood (4, 5). Alternative splicing may generate multiple isoforms with deletions or substitutions in the N-terminal and collagen-like domains (6). CL-K1 is widely expressed, notably in renal proximal tubules, bronchial glands, and gastrointestinal mucosa (5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature human CL-K1 shares 93% amino acid sequence identity with mouse and rat CL-K1. Within the CRD, human CL-K1 shares 55% aa sequence identity with CL-L1 and 26% - 33% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.
References:
Gupta, G. and A. Surolia, 2007, Bioessays 29:452.
van de Wetering, J.K. et al., 2004, Eur. J. Biochem. 271:1229.
Holmskov, U. et al., 2003, Annu. Rev. Immunol. 21:547.
Keshi, H. et al., 2006, Microbiol. Immunol. 50:1001.
Motomura, W. et al., 2008, J. Histochem. Cytochem. 56:243.
SwissProt # Q9BWP8.
Long Name:
Collectin Kidney 1
Entrez Gene IDs:
78989 (Human); 71693 (Mouse)
Alternate Names:
CDC28/CDC2-like kinase; CDC-like kinase 1CLKEC 2.7.12.1; CLK/STY; CLK1; CL-K1; COLEC11; dual specificity protein kinase CLK1; protein tyrosine kinase STY; STY
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