• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED    ₅₀ for this effect is 25-100 ng/mL.

• Source

  Human embryonic kidney cell, HEK293-derived

  Human PANP (Ser76-Pro178) Accession # Q8IYJ0	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser76 & Val80

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  37 kDa (monomer)

• SDS-PAGE

  58-69 kDa, reducing conditions

Background: PANP

PILR‑alpha associated neural protein (PANP) is an approximately 30 kDa type 1 transmembrane glycoprotein that contains a 147 amino acid (aa) extracellular domain (ECD) and an 83 aa cytoplasmic domain (1). Within the ECD, human PANP shares 93% aa sequence identity with mouse and rat PANP. Alternative splicing may generate an additional isoform with a 6 aa truncation at the C‑terminus. PANP is preferentially expressed in the central nervous system (1). It binds to PILR‑alpha, an inhibitory receptor that is expressed on dendritic cells, neutrophils, and macrophages (1, 2). PILR‑alpha additionally binds to CD99, NPDC‑1, CL‑P1/COLEC12, and glycoprotein B of Herpes simplex virus type 1 (3‑5). PILR‑alpha binding requires the presence of sialylated O‑linked carbohydrates on each of these ligands, including PANP (1, 3, 4, 6). The   in cis association of PILR‑alpha with sialylated molecules on the neutrophil surface inhibits beta integrin activation and neutrophil extravasation during inflammatory responses (7).

• References:

1. Kogure, A. et al. (2011) Biochem. Biophys. Res. Commun. 405:428.

2. Fournier, N. et al. (2000) J. Immunol. 165:1197.

3. Wang, J. et al. (2008) J. Immunol. 180:1686.

4. Sun, Y. et al. (2012) J. Biol. Chem. 287:15837.

5. Satoh, T. et al. (2008) Cell 132:935.

6. Wang, J. et al. (2009) J. Virol. 83:13042.

7. Wang, J. et al. (2013) Nat. Immunol. 14:34.

• Long Name:

  PILR alpha Associated Neural Protein

• Entrez Gene IDs:

  196500 (Human); 319352 (Mouse); 312711 (Rat)

• Alternate Names:

  C12orf53; chromosome 12 open reading frame 53; PANP; PIANP; PILR alpha associated neural protein