• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce IFN-gamma secretion by mouse splenocytes. The ED    ₅₀ for this effect is 0.4-2 μg/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Trp49-Leu174 (Gly136Asp), with an N-terminal HA tag

• Accession #

• N-terminal Sequence    
  Analysis

  Tyr

• Predicted Molecular Mass

  16 kDa

• SDS-PAGE

  19-30 kDa, reducing conditions

Data Images

Background: CLEC-2A

CLEC-2A, also called keratinocyte-associated C-type lectin (KACL), is a type 2 transmembrane glycoprotein member of the C-type lectin-like receptor (CTLR) family. CLEC-A2 is part of the subgroup of CLEC-2 proteins that also includes CLEC-2B/AICL, CLEC-2D/LLT, and CD69/CLEC-2C, all of which are encoded by the natural killer gene complex (NKC) (1, 2). CLEC-2A is composed of a 126 amino acid (aa) carboxy terminal extracellular C-type lectin-like domain (CTLD), a 21 aa transmembrane domain and a 27 aa amino terminal cytoplasmic domain. The CTLD folds into a compact structure with two alpha -helices and two antiparallel beta -sheets stabilized by three conserved intramolecular disulfide bonds (3). CLEC-2 proteins act as ligands and interact with NKRP1 receptors which also are CTLR and encoded by the NKC. The crystal structure of the complex shows CLEC-2A as a non-disulfide linked homodimer that symmetrically binds two NKp65 monomers. Interaction occurs via the membrane-distal surface of the CTLD in a head-to-head orientation (4). Five key residues for CLEC-2A binding to NKp65 are conserved or conservatively substituted among CLEC-2 proteins. Thus, the CLEC-2A-NKp65 interaction is proposed as a model for all NKRP1-CLEC-2 complexes (4). Orthologs for NKp65 and CLEC-2A are present in chimpanzee, rhesus macaque and cow but have not been described in rodents (5). Whereas CLEC-2B/AICL, CLEC-2D/LLT, and CD69/CLEC-2C are generally expressed on hematopoietic cells, CLEC-2A expression is restricted to keratinocytes. In vitro, the NKp65-CLEC-2A interaction will trigger natural killer cell cytotoxicity and the release of proinflammatory cytokines (6). Thus, CLEC-2A facilitates NKp65-mediated immunosurveillance of keratinocytes (6).

• References:

1. Spreu, J. et al. (2007) Immunogenetics 59:903.

2. Yokohama, W.M. et al. (2003) Nat.Rev.Immunol. 3:304.

3. Zelensky, A.N. et al. (2005) FEBS J. 272:6179.

4. Li, Y. et al. (2013) Proc. Natl. Acad. Sci. USA 110:11505.

5. Vogler, I. et al. (2011) J. Innate Immun. 3:227.

6. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.

• Long Name:

  C-type Lectin Domain Family 2, Member A

• Entrez Gene IDs:

  387836 (Human)

• Alternate Names:

  CLEC2A C-type lectin domain family 2, member A ; CLEC2A; CLEC-2A; KACL; PILAR; UNQ5792