• Purity

  >80%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED    ₅₀ for this effect is 2.5-12.5 μg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # ).

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human Lgr6 (Ala25-Ala567) Accession # Q9HBX8	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  87 kDa

• SDS-PAGE

  90-108 kDa, reducing conditions

Data Images

Background: Lgr6

Lgr6 (leucine‑rich repeat G protein‑coupled receptor 6) is an approximately 100 kDa 7‑transmembrane glycoprotein in the Lgr family of cell surface receptors. Lgrs has been identified as a marker for specific types of stem cells in tissues that are subject to constant self-renewal such as skin, gastrointestinal tract, and lungs (1, 2). The 544 amino acid (aa) N-terminal extracellular domain (ECD) of mature human Lgr6 contains 15 LRRs flanked by N-terminal and C-and C-terminal LRR domains. The ECD is followed by a 7-TM region and a 137 aa C-terminal cytoplasmic domain (3). Within the N-terminal ECD, human Lgr6 shares 92% and 93% aa sequence identity with mouse and rat Lgr6, respectively. Alternative splicing generates an additional isoform with a substitution for the LRR N-terminal domain and a deletion of LRR3-6. Lgr6 is expressed on hair follicle stem cells that give rise to sebaceous glands, interfollicular epidermis, and hair follicles (4-6). It is also expressed on alveolar stem cells that differentiate into lung epithelium (7). It is often up-regulated in human gastric cancers (8). Lgr6 binds to R‑Spondins 1-4 and can enhance Wnt/beta-Catenin signaling through phosphorylation of the Wnt co-receptor LRP6 (9, 10).

• References:

1. de Lau, W. et al. (2014) Genes Dev. 28:305.

2. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.

3. Hsu, S.Y. et al. (2000) Mol. Endocrinol. 14:1257.

4. Snippert, H.J. et al. (2010) Science 327:1385.

5. Liao, X.-H. and H. Nguyen (2014) Exp. Dermatol. 23:195.

6. Nath, M. et al. (2011) Cell Tissue Res. 344:435.

7. Oeztuerk-Winder, F. et al. (2012) EMBO J. 31:3431.

8. Steffen, J.S. et al. (2012) Virchows Arch. 461:355.

9. Gong, X. et al. (2012) PLoS ONE 7:e37137.

10. de Lau, W. et al. (2011) Nature 476:293.

• Long Name:

  Leucine-rich Repeat Containing G Protein-coupled Receptor 6

• Entrez Gene IDs:

  59352 (Human); 329252 (Mouse); 498233 (Rat)

• Alternate Names:

  FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631