• Purity

  >90%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP3/PGRP-I alpha that produces 50% optimal binding response is 10-60 ng/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Trp18-His341, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Trp18

• Structure / Form

  Disulfide-linked Homodimer

• Predicted Molecular Mass

  37 kDa

• SDS-PAGE

  34-40 kDa, reducing conditions

Background: PGLYRP3/PGRP-I alpha

PGLYRP3, also known as PGRP-1 alpha, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP3 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP3 contains two nonidentical PGRP domains, and it shares 74% and 75% amino acid sequence identity with mouse and rat PGLYRP3, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP3 is directly cytotoxic against pathogenic and nonpathogenic Gram-positive and Gram-negative bacteria (3, 5). Its bactericidal activity requires physiological concentrations of Zn²⁺ (5). PGLYRP3 has also been shown to have an anti-inflammatory role in intestinal epithelial cells (6). PGLYRP3 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (7-9). In humans, PGLYRP3 single nucleotide polymorphisms have been associated with inflammatory bowel disease (10).

• References:

1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.

2. Park, S.Y. et al. (2011) PLoS One 6:e24961.

3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.

4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.

5. Wang, M. et al. (2007) J. Immunol. 178:3116.

6. Zenhom, M. et al. (2012) Immunobiology 217:412.

7. Park, S.Y. et al. (2011) PLoS One 6:e24961.

8. Saha, S. et al. (2010) Cell Host Microbe 8:147.

9. Jing, X. et al. (2014) J. Immunol. 193:3055.

10. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.

• Long Name:

  Peptidoglycan Recognition Protein Intermediate, Alpha/Peptidoglycan Recognition Protein 3

• Entrez Gene IDs:

  114771 (Human); 242100 (Mouse); 499658 (Rat)

• Alternate Names:

  peptidoglycan recognition protein 3; peptidoglycan recognition protein I alpha; Peptidoglycan recognition protein I-alpha; Peptidoglycan recognition protein intermediate alpha; peptidoglycan recognition protein-I-alpha; PGLYRP3; PGLYRPIalphaMGC149197; PGRP1A; PGRPI alpha; PGRP-I alpha; PGRPIA; PGRP-Ialpha; PGRPIAPGRP-I-alpha