• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED    ₅₀ for this effect is 0.1-0.3 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived Met28-Leu206, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Gln52 predicted, no results obtained, sequencing might be blocked.

• Predicted Molecular Mass

  19.5 kDa

• SDS-PAGE

  35 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: IL-24

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4⁺ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL‑24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL-12 and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL‑20 R alpha and IL-20 R beta, which is shared with IL-19 and IL‑20. The second complex is a combination of IL‑22 R and IL-20 R beta, which is shared with IL-20.

• References:

1. Jiang, H. et al. (1995) Oncogene 11:2477.

2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.

3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.

4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.

5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

6. Chen, J. et al. (2003) Molec. Ther. 8:220.

• Long Name:

  Interleukin 24

• Entrez Gene IDs:

  11009 (Human); 93672 (Mouse); 170819 (Rat)

• Alternate Names:

  C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein