• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED    ₅₀ for this effect is typicallu 0.025-0.125 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Ser30-Tyr693, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ser30

• Predicted Molecular Mass

  73 kDa

• SDS-PAGE

  83-123 kDa, reducing conditions

Background: Protocadherin gamma C3

Protocadherin  gamma  C3 is a member of the gamma  subgroup of clustered protocadherins (1). Like other gamma  protocadherins, mature Protocadherin  gamma  C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin  gamma  C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA  ²⁺ -dependent homophilic interaction (4). MMP-mediated shedding of gamma  protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin  gamma  C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma  gene cluster is embryonic lethal in mice (9). Protocadherin  gamma  C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin  gamma  gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma  isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma  gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma  gene cluster results in dendritic arborization defects (16). The protocadherin gamma  subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).

• References:

1. Yagi, T. (2008) Dev. Growth Differ. 50:S131.

2. Sano, K. et al. (1993) EMBO J. 12:2249.

3. Kohmura, N. et al. (1998) Neuron 20:1137.

4. Obata, S. et al. (1995) J. Cell Sci. 108:3765.

5. Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.

6. Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.

7. Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.

8. Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.

9. Wang, X. et al. (2002) Neuron 36:843.

10. Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.

11. Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.

12. Lefebvre, J.L. et al. (2008) Development 135:4141.

13. Prasad, T. et al. (2008) Development 135:4153.

14. Lin, C. et al. (2010) J. Biol. Chem. 285:41675.

15. Chen, W.V. et al. (2012) Neuron 75:402.

16. Garrett, A.M. et al. (2012) Neuron 74:269.

17. Lobas, M.A. et al. (2012) J. Neurochem. 120:913.

18. Ledderose, J. et al. (2013) Sci. Rep. 3:1514.

• Entrez Gene IDs:

  5098 (Human); 93706 (Mouse); 116782 (Rat)

• Alternate Names:

  Cadherin-Like 2; PC43; PC-43; PCDH2; PCDH-GAMMA-C3; PCDHGC3; protocadherin 2; Protocadherin 43; Protocadherin gamma C3; protocadherin gamma subfamily C, 3; protocadherin gamma-C3; Protocadherin-2; protocadherin-43