• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED    ₅₀ for this effect is 0.6-3 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Gln21-His462 (Leu220Phe) with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Gln21

• Predicted Molecular Mass

  49 kDa

• SDS-PAGE

  67-81 kDa, reducing conditions

Background: LRIT2

Leucine-rich Repeat, Immunoglobulin-like domain and Transmembrane domain-containing protein 2 (LRIT2), also known as leucine-rich repeat protein 22 (LRRC22), is a 550 amino acid (aa) type I transmembrane protein. Its extracellular domain (ECD) contains one LRRNT, LRRCT, Ig-like, and Fibronectin type-III domain, and four leucine-rich repeats (LRRs). Within the ECD, human LRIT2 shares approximately 75% aa sequence identity with the mouse and rat orthologs. The LRR is a structural motif consisting of 20 to 30 aa repeated 2 to 45 times that forms a hydrophobic horseshoe shape with a parallel beta  sheet on the concave side and helical elements on the convex side (1-4). All LRRs are composed of a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif (1-4). LRR motifs mediate protein-protein interactions in a variety of proteins including tyrosine kinase receptors, hormone receptors, extracellular matrix-binding proteins, cell-adhesion molecules, and bacterial virulence factors (1, 3). Proteins containing LRRs play important roles in many biologically processes such as mammalian and neural development, cell polarization, gene expression, and apoptosis (2).

• References:

1. Kobe, B. and J. Deisenhofer (1994) Trends Biochem. Sci. 19:415.

2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725.

3. Enkhbayar, P. et al. (2004) Proteins 54:394.

4. Bella, J. et al. (2008) Cell. Mol. Life Sci. 65:2307.

• Long Name:

  Leucine-Rich Repeat, Immunoglobulin-Like And Transmembrane Domains 2

• Entrez Gene IDs:

  340745 (Human); 239038 (Mouse)

• Alternate Names:

  immunoglobulin-like domain and transmembrane domain-containing protein 2; leucine rich repeat containing 22; leucine-rich repeat, immunoglobulin-like and transmembrane domains 2; leucine-rich repeat-containing protein 22; LRIT2; LRRC22; PAL2