Human Cytokeratin 8 Alexa Fluor 488 MAb (Clone LP3K) 100 TESTS

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Human Cytokeratin 8 Alexa Fluor 488 MAb (Clone LP3K) 100 TESTS信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects human Cytokeratin 8 in flow cytometry.

    • Source

      Monoclonal Mouse IgG    1 Clone # LP3K

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      SV-40 transformed human neonatal keratinocyte extract

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 488

    Applications


    • Recommended    
      Concentration

      Sample

    • Intracellular Staining by Flow Cytometry

      5 µL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Intracellular Staining by Flow Cytometry      
         

    Detection of Cytokeratin 8 in HT‑29 Human Cell Line by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (upper panel, negative control) and HT‑29 human colon adenocarcinoma cell line (lower panel, positive control) were stained with Mouse Anti-Human Cytokeratin 8 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3165G, filled histograms) or isotype control antibody (Catalog # , open histograms). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: Cytokeratin 8

    Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.

    • Entrez Gene IDs:

      3856 (Human); 16691 (Mouse); 25626 (Rat)

    • Alternate Names:

      CARD2; CK8; CK-8; CYK8cytokeratin 8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8cytokeratin-8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8




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