• Species Reactivity

  Mouse

• Specificity

  Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human MMR is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206    
  Leu19-Ala1388    
  Accession # Q2HZ94

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca²⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

• References:

1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 

2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 

3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 

4. Harris, N. et al. (1992) Blood 80:2363.

5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.

6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.

7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.

8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

• Long Name:

  Macrophage Mannose Receptor

• Entrez Gene IDs:

  4360 (Human); 17533 (Mouse); 291327 (Rat)

• Alternate Names:

  CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1