• Species Reactivity

  Human

• Specificity

  Detects human Transglutaminase 3/TGM3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) TGM2, rhTGM4, and rhTGM7 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 3/TGM3
  Ala2-Glu693
  Accession # NP_003236

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 3/TGM3

Transglutaminase 3 (TG3), also known as epidermal Transglutaminase (Tgase E), belongs to the family of Transglutaminase enzymes that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). TG3 is involved in the formation of the cornified envelope in skin keratinocytes (4). It functions to cross-link structural proteins during epidermal terminal differentiation. TG3 has been implicated as the dominant autoantigen in dermatitis herpetiformis (5). TG3 activation requires proteolysis of the 77 kDa zymogen into two fragments of approximately 50 and 27 kDa to form the active enzyme (1).

• References:

1. Kim, I.G. et al. (1993) J. Biol. Chem. 268:12682.

2. Griffin, M. et al. (2002) Biochem. J. 368:377.

3. Lorand, L. and R.M. Graham (2003) Nat. Rev. Mol. Cell Biol. 4:140.

4. Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.

5. Sardy, M. et al. (2002) J. Exp. Med. 195:747.

• Entrez Gene IDs:

  7053 (Human); 21818 (Mouse)

• Alternate Names:

  EC 2.3.2.13; MGC126250; protein-glutamine gamma-glutamyltransferase E; TG(E); TG3; TGase E; TGase-3; TgaseE; TGEMGC126249; TGM3; transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 3; Transglutaminase E; transglutaminase-3