• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects endogenous human, mouse and rat PI 3-Kinase p55 gamma in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human PI 3-Kinase p55 gamma    
  Lys251-Gly378    
  Accession # Q92569

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI 3-Kinase regulatory subunit gamma and p55  ^PIK) is a 55-57 kDa member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55 gamma /p55  ^PIK should not be confused with the 55 kDa alpha splice variant of the p85 alpha  gene (75% amino acid [aa] identity). Human p55 gamma  is 461 aa in length and contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452).

• Long Name:

  PI 3-Kinase p55 gamma

• Entrez Gene IDs:

  8503 (Human); 18710 (Mouse); 60664 (Rat)

• Alternate Names:

  p55PIK; PI 3Kinase p55 gamma; PI 3-Kinase p55 gamma; PIK3R3