• Species Reactivity

  Human

• Specificity

  Detects human Clathrin Heavy Chain 1/CHC17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CHC-22 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Clathrin Heavy Chain 1/CHC17    
  Trp1521-Met1675    
  Accession # Q00610

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Clathrin Heavy Chain 1/CHC17

Clathrin Heavy Chain 1/CHC17 (clathrin heavy chain 17; also CHC, CLTCL2 and CLTC) is a 170‑180 kDa member of the clathrin heavy chain family of molecules. It is widely expressed, and serves at least two functions. First, it interacts with TACC3 and chTOG to form a complex that crosslinks microtubules making up K‑fibers, and second, three Clathrin Heavy Chain 1/CHC17 molecules form a triskelion (Greek for "3 legged") structure with three clathrin light chains to create the polyhedral coat of coated pits. Human Clathrin Heavy Chain 1/CHC17 is 1675 amino acids (aa) in length. It contains an N‑terminal domain that mediates protein‑protein interactions (aa 2‑479), a flexible linker region (aa 480‑523), a distal segment (aa 524‑638), a proximal region that interacts noncovalently with one CHC light chain (aa 639‑1549), and a trimerization domain that mediates the formation of a noncovalent triskelion structure (aa 1550‑1675). Although the predicted MW of Clathrin Heavy Chain 1/CHC17 is 191 kDa, it runs anomalously at 180 kDa in SDS‑PAGE. Multiple utilized phosphorylation and acetylation sites exist. There are multiple potential splice forms. One shows a four aa insertion after Lys83, a second shows a seven aa insertion after Asp1611, and a third contains a four aa substitution for aa 1636‑1675. Over aa 1521‑1675, human and mouse Clathrin Heavy Chain 1/CHC17 are identical in aa sequence.

• Long Name:

  Clathrin Heavy Chain on Chromosome 17

• Entrez Gene IDs:

  1213 (Human); 67300 (Mouse); 54241 (Rat)

• Alternate Names:

  CHC; CHC17; Clathrin Heavy Chain 1; Clathrin heavy chain on chromosome 17; clathrin, heavy chain (Hc); clathrin, heavy chain; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH17; CLH-17; CLTC; CLTCL2; Hc; KIAA0034