• Assay Type

  Solid Phase Sandwich ELISA

• Format

  96-well strip plate

• Assay Length

  4 hours 40 mins (after plate preparation)

• Sample Type & Volume Required

  Cell lysates (100 µL)

• Range

  312.00 - 20,000 pg/mL

• Sufficient Materials

  Kits available for two, five, or fifteen 96-well plates*

• Specificity

  Please see the

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

• Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time

• Development protocols are provided to guide further assay optimization

• Assay can be customized to your specific needs

• Available in 2, 5, and 15- (96-well) plate pack sizes

• Economical alternative to Western blot

Kit Content

• Capture Antibody

• Conjugated Detection Antibody

• Calibrated Immunoassay Standard or Control

• Streptavidin-HRP

Other Reagents Required

  PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na  ₂HPO  ₄, 1.5 mM KH  ₂O  ₄, pH 7.2 - 7.4, 0.2 µm filtered  
 
  Wash Buffer: (Catalog # ), or equivalent  
 
  Lysis Buffer*  
 
  IC Diluent*

Blocking Buffer*  
 
  Substrate Solution: 1:1 mixture of Color Reagent A (H  ₂O  ₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # )  
 
  Stop Solution: 2 N H  ₂SO  ₄ (Catalog # )  
 
  Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # ), or equivalent  
 
  Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent  
 

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta-Catenin

beta-Catenin, also known as CTNNB1, is a cadherin-associated protein that is involved in the regulation of cell adhesion. This multifunctional protein is also implicated in the canonical Wnt signal transduction pathway, acting as a transcriptional co-activator. Nuclear beta-Catenin activates the TCF/LEF family of transcription factors, inducing the transcription of pro-mitotic genes.

• Entrez Gene IDs:

  1499 (Human); 12387 (Mouse); 84353 (Rat);

• Aliases:

  bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923