• Assay Type

  Solid Phase Sandwich ELISA

• Format

  96-well strip plate

• Sample Type & Volume Required

  Cell lysates (100 µL)

• Sufficient Materials

  Kits available for two, five, or fifteen 96-well plates*

• Specificity

  Please see the

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated

Product Features

• Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time

• Development protocols are provided to guide further assay optimization

• Assay can be customized to your specific needs

• Available in 2, 5, and 15-(96-well) plate pack sizes

• Economical alternative to Western blot

Kit Content

• Capture Antibody

• Conjugated Detection Antibody

• Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # ), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # )

Stop Solution: 2 N H₂SO₄ (Catalog # )

Microplates: R&D Systems (Catalog # ), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Insulin R/CD220

Insulin Receptor (INSR, CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases. The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor. All receptor combinations bind Insulin, IGF-I, or IGF-II, but with differing affinities. Insulin R signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. Mutations in INSR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome).

• Entrez Gene IDs:

  3643 (Human); 16337 (Mouse);

• Long Name:

  Insulin Receptor

• Aliases:

  CD 220; CD220 antigen; CD220; EC 2.7.10; EC 2.7.10.1; HHF5; INSR; Insulin R; insulin receptor; InsulinR; IR