• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse SSEA-1.

• Source

  Monoclonal Mouse IgM Clone # MC-480

• Immunogen

  F9 mouse teratocarcinoma stem cells

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  D3 mouse embryonic stem cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

• References:

1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.

2. Fox, N. et al. (1983) Cancer Res. 43:669.

• Long Name:

  Stage-specific Embryonic Antigen-1

• Alternate Names:

  alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1