• Species Reactivity

  Human

• Specificity

  Detects human OCIL/CLEC2d.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 402659

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human OCIL/CLEC2d    
  Ser57-Val191    
  Accession # Q9UHP7

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: OCIL/CLEC2d

Human OCIL (Osteoclast Inhibitory Lectin), also known as LLT1 and CLEC-2D, is a member of the NK cell receptor group of the C-type lectin superfamily. It is a type II transmembrane protein 191 amino acids (aa) in length that contains a C-terminal 132 aa extracellular domain (ECD). Within the ECD lies a C-type lectin domain (aa 75-186). There are at least two alternate splice forms of OCIL. Both exhibit splicing in the ECD. One shows a 40 aa substitution for the C-terminal 37 amino acids. A second shows a 3 aa substitution for the C-terminal 72 amino acids. The ECD of human OCIL is 49% and 50% aa identical to the ECD in mouse and rat OCIL ECD, respectively. The molecule is found on hematopoietic cells, osteoblasts and chondrocytes. It binds sulfated GAGs and NKR-P1B and D receptors, and blocks osteoclast formation.

• Long Name:

  Osteoclast Inhibitory Lectin

• Entrez Gene IDs:

  29121 (Human); 93694 (Mouse)

• Alternate Names:

  CLAXLLT-1; CLEC2D; C-type lectin domain family 2 member D; C-type lectin domain family 2, member D; C-type lectin related f; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT1; LLT1Osteoclast inhibitory lectin; OCIL; OCILmember D