• Species Reactivity

  Human

• Specificity

  Detects human ErbB4/Her4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) EGF R, rhErbB2, or rhErbB3 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 182818

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human ErbB4/Her4    
  Glu26-Arg649    
  Accession # Q15303

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB4/Her4

ErbB4, also called Her4 (human epidermal growth factor receptor 4), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme, ADAM17) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25 aa signal sequence, a 626 aa extracellular domain, a 24 aa transmembrane region, and a 633 aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.

• References:

1. Plowman, G.D. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1746. 

2. Elenius, K. et al. (1997) J. Biol. Chem. 272:26761.

3. Elenius, K. et al. (1999) Oncogene 18:2607.

4. Rio, C. et al. (2000) J. Biol. Chem. 275:10379.

5. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.

6. Sundaresan, S. et al. (1998) Endocrinology 139:4756.

7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.

8. Schlessinger, J. (2000) Cell 103:211.

9. Daly, R.J. (1999) Growth Factors 16:255.

• Long Name:

  Receptor Tyrosine Protein Kinase ErbB4

• Entrez Gene IDs:

  2066 (Human); 13869 (Mouse); 59323 (Rat)

• Alternate Names:

  avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; EC 2.7.10; EC 2.7.10.1; ErbB4; HER4; HER4MGC138404; p180erbB4; Proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)