• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL‑6 R alpha in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human IL-6 R alpha is observed.

• Source

  Monoclonal Rat IgG2a Clone # 255821

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha    
  Leu20-Glu357    
  Accession # P22272

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse CD3    ⁺ splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6 R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). Mature mouse IL-6 R alpha is a type I transmembrane protein of 460 amino acids (aa) that contains a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSxWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds  IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

• References:

1. Yamasaki, K. et al. (1988) Science 241:825.

2. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.

3. Hibi, M. et al. (1990) Cell 63:1149.

4. Saito, M. et al. (1992) J. Immunol. 148:4066.

5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.

6. Jones, S.A. et al. (2001) FASEB J. 15:43.

7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

• Long Name:

  Interleukin 6 Receptor alpha

• Entrez Gene IDs:

  3570 (Human); 16194 (Mouse)

• Alternate Names:

  CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra