详细说明
Species Reactivity
Human
Specificity
Detects human S1P 1/EDG‑1 by flow cytometry.
Source
Monoclonal Mouse IgG 2B Clone # 218713
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P 1/EDG‑1
Met1-Ser382
Accession # P21453Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with human S1P1/EDG-1 were stained with Mouse Anti-Human S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2016G, filled histogram) or Mouse IgG2B Alexa Fluor® 488‑conjugated Isotype Control Antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P1/EDG-1
S1P 1, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P 1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.
Long Name:
Endothelial Differentiation Gene 1
Entrez Gene IDs:
1901 (Human); 13609 (Mouse)
Alternate Names:
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1