• Species Reactivity

  Human

• Specificity

  Detects human CD229/SLAMF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse CD229 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 249936

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CD229/SLAMF3    
  Lys48-Lys454    
  Accession # Q9HBG7

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD229/SLAMF3

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CD229 consists of a 407 amino acid (aa) extracellular domain (ECD) with two Ig‑like V-set and two Ig‑like truncated C2-set domains. It also shows a 22 aa transmembrane segment, and a 179 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). The ECD of human CD229 shares 57%‑59% aa sequence identity with mouse and rat CD229. Within the first two Ig‑like domains that are common to all SLAM proteins, human CD229 shares 24%‑39% aa sequence identity with human 2B4, BLAME, CD2F-10, CD84, CRACC, NTB-A, and SLAM. Alternate splicing generates two additional isoforms that lack the juxtamembrane Ig‑like domain or short cytoplasmic region (2). CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells (2‑6). Homophilic binding between CD229 molecules is mediated by the N-terminal Ig‑like domain (7). Human and mouse CD229 exhibit cross-species binding (7). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell‑B cell contact (7). Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization (8, 9). In addition, there are two ITSMs which mediate phosphorylation-dependent CD229 association with SAP and SHP-2 (10). These four tyrosine-containing motifs are intact in the described CD229 splice variants. CD229 knockout mice show minimally impaired immune responses, suggesting functional redundancy with other molecules (11).

• References:

1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.

2. de la Fuente, M.A. et al. (2001) Blood 97:3513. 

3. Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.

4. Romero, X. et al. (2004) Tissue Antigens 64:132. 

5. Hogarth, P.M. et al. (1980) Immunogenetics 11:65. 

6. Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.

7. Romero, X. et al. (2005) J. Immunol. 174:7033.

8. Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.

9. Martin, M. et al. (2005) J. Immunol. 174:5977.

10. Sayos, J. et al. (2001) Blood 97:3867.

11. Graham, D.B. et al. (2006) J. Immunol. 176:291.

• Long Name:

  SLAM Family Member 3

• Entrez Gene IDs:

  4063 (Human); 17085 (Mouse)

• Alternate Names:

  CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9