• Species Reactivity

  Human

• Specificity

  Stains human Claudin-1 transfectants but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Rat IgG    _2A Clone # 421203

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  CHO Chinese hamster ovary cell line transfected with human Claudin-1    
  Met1-Val211    
  Accession # O95832

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-1

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

• Entrez Gene IDs:

  9076 (Human); 12737 (Mouse); 65129 (Rat)

• Alternate Names:

  claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1