详细说明
| Replacement Information |
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重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
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| JNK/SAPK1 (Thr183/Tyr185) | H, M | Mplex | Luminex xMAP |
| Description | |
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| Catalogue Number | 46-613MAG |
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| Description | MILLIPLEX MAP Phospho JNK/SAPK1 (Thr183/Tyr185) Magnetic Bead MAPmate - Cell Signaling Single Plex |
| Materials Required but Not Delivered | • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602) |
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| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Panel Type | Cell Signaling |
| Applications | |
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| Application | MAPmate single plexes allow you to design your own cell signaling panel. The analyte available for this kit is JNK/SAPK1 (Thr183/Tyr185). |
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| Application Notes | • An overnight (4°C) incubation is recommended for best results • This assay requires 25 µL diluted cell lysate per well • Please consult the buffer compatibility chart or protocol for appropriate buffer choice • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg) |
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| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. |
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| UniProt Summary | FUNCTION: SwissProt: P45983 # JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. COFACTOR: Magnesium. SIZE: 427 amino acids; 48296 Da SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity). DOMAIN: SwissProt: P45983 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. |
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| Material Size | 96-well plate |
| Material Package | 96-well Plate |
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