别名:   NP NPO

产品名称

Biochem/physiol Actions

Neuropoietin is a newly identified member of the IL-6 cytokine family. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 2.5 mM Tris, pH 10.2 + 0.5 mM DTT.

General description

Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-a component, gp 130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues.

Sequence

MAPISPSEPI GQAYSLALYM QKNTSALLQT YLQHQGSPFS DPGFSAPELQ LSTLPSAAVS FKTWHAMEDA ERLSRAQGAF LALTQHLQLV GDDQSYLNPG SPILLAQLGA ARLRAQGLLG NMAAIMTALG LPIPPEEDTL GFVPFGASAF ERKCRGYIVT REYGHWTDRA VRDLALLKAK YSA

性质

recombinant	expressed in E. coli
assay	≥98% (HPLC)
	≥98% (SDS-PAGE)
form	lyophilized
mol wt	mol wt 19.8 kDa
impurities	<0.1 EU/μg endotoxin, tested
suitability	cell culture tested
UniProt accession no.	P83714
shipped in	wet ice
storage temp.	?20°C
Gene Information	mouse ... CTF2(244218)