质量水平

500

描述

for research or for further manufacturing use

无菌性

sterile-filtered

形式

dry powder

储存条件

protect from light

应用

cell culture | mammalian: suitable

pH值（酸碱度）

7.1-7.7

储存温度

2-8°C

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相关类别

Bioprocessing Cell Culture Media

应用

EX-CELL^® 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR^- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR^- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

注意

请勿冰冻。避光保存。培养基中的几种成分不耐光，不应长时间暴露在光照下。

数量

Formulated to contain 21.2 grams of powder per liter of medium.

附注

Ex-Cell 302 has also been used successfully with BHK-21 cells and chicken embryo-related (CER) cell lines.

法律信息

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC