概述

• 产品名称Anti-14-3-3 epsilon抗体[EPR3918]
     

• 描述

  兔单克隆抗体[EPR3918] to 14-3-3 epsilon

• 经测试应用ICC/IF,WB,Flow Cyt,IHC-Fr

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in Human 14-3-3 epsilon (UniProt ID: P62258).

• 阳性对照    

• Human fetal brain, SH-SY5Y, 293T and SW480 cell lysates and HeLa cells

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR3918

• 同种型IgG

• 研究领域

• Stem Cells

• Signaling Pathways

• Wnt

• 14-3-3

• Neuroscience

• Cell Type Marker

• Neuron marker

• Synapse marker

• Signal Transduction

• Adapters

• Cytoplasmic

• <a javascript:void(0)>Neuroscience

• Neurology process

• Neural Signal Transduction

Anti-14-3-3 epsilon antibody [EPR3918] 图像

• 

  Flow Cytometry - Anti-14-3-3 epsilon antibody [EPR3918] (ab92311)          

     Overlay histogram showing SH-SY5Y cells stained with ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.  

• 

  Western blot - 14-3-3 epsilon antibody [EPR3918] (ab92311)          

     All lanes : Anti-14-3-3 epsilon antibody [EPR3918] (ab92311) at 1/1000 dilution
  
  Lane 1 : Human fetal brain cell lysate
  Lane 2 : SH-SY5Y cell lysate
  Lane 3 : 293T cell lysate
  Lane 4 : SW480 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 29 kDa
   

• 

  Immunocytochemistry/ Immunofluorescence - 14-3-3 epsilon antibody [EPR3918] (ab92311)          

     ab92311 at 1/100 dilution staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.