概述

• 产品名称Anti-14-3-3 Theta + Tau抗体[EPR5123]

• 描述

  兔单克隆抗体[EPR5123] to 14-3-3 Theta + Tau

• 经测试应用ICC/IF,WB,Flow Cyt

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 14-3-3 Theta + Tau (N terminal). The exact sequence is proprietary.

• 阳性对照    

• HeLa, 293T, A549 and A431 cell lysates; A549 cells

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 解离常数（K_D） K_D = 6.09 x 10 ^-10 M Learn more about K_D

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR5123

• 同种型IgG

• 研究领域

• Stem Cells

• Signaling Pathways

• Wnt

• 14-3-3

• Signal Transduction

• Adapters

• Cytoplasmic

Anti-14-3-3 Theta + Tau antibody [EPR5123] 图像

• 

  Western blot - Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909)          

     All lanes : Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909) at 1/10000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : 293T cell lysate
  Lane 3 : A549 cell lysate
  Lane 4 : A431 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 28 kDa
  Observed band size : 28 kDa
   

• 

  Flow Cytometry - Anti-14-3-3 Theta + Tau antibody [EPR5123] (ab124909)          

     Flow cytometric analysis of permeablized A549 cells using ab124909 at 1/500 dilution (red) or a rabbit IgG negative control (green).  

• 

  Other-Anti-14-3-3 Theta + Tau antibody [EPR5123](ab124909)          

     Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D