概述

• 产品名称Anti-AMPK beta 1 (phospho S181)抗体[EP2181Y]
     

• 描述

  兔单克隆抗体[EP2181Y] to AMPK beta 1 (phospho S181)

• 经测试应用WB,IHC-P,ICC

• 种属反应性

  与反应: Human    
   

• 免疫原

  A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta 1.

• 阳性对照    

• Human brain tissue lysate (cells untreated or treated with AP), HeLa cells and human skeletal muscle tissue

• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP2181Y

• 同种型IgG

• 研究领域

• Metabolism

• Pathways and Processes

• Redox metabolism

• Fatty acid oxidation

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Energy transfer pathways

• Integration of energy

• Metabolism

• Pathways and Processes

• Metabolic signaling pathways

• Lipid and lipoprotein metabolism

• Fatty acids

• Cancer

• Cancer Metabolism

• Metabolic signaling pathway

• Integration of energy metabolism

• Cardiovascular

• Lipids / Lipoproteins

• Fatty Acids

• Metabolism

• Signal Transduction

• Metabolism

• Lipid metabolism

• <a javascript:void(0)>Signal Transduction

• Signaling Pathway

• Lipid Signaling

• Lipid Kinases

Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] 图像

• 

  Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)          

     All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/20000 dilution
  
  Lane 1 : human brain tissue lysate (cells were untreated)
  Lane 2 : human brain tissue lysate (cells were treated with AP)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 30 kDa
  Observed band size : 32 and 45 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunocytochemistry/ Immunofluorescence - AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)          

     Immunofluorescent staining of HeLa cells using ab68206 at 1/100-1/250 dilution.  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)          

     Immunohistochemical analysis of paraffin-embedded human skeletal muscle using ab68206 at 1/100-1/250 dilution.