详细说明
概述
产品名称Anti-ANP32C抗体[EPR13354-70R]
描述
兔单克隆抗体[EPR13354-70R] to ANP32C
经测试应用WB,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ANP32C aa 100-200. The exact sequence is proprietary.
Database link: O43423阳性对照
MCF-7, PC-3, BxPC-3, C6, Raw264.7 and NIH/3T3 cell lysates.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13354-70R
同种型IgG
研究领域
Cancer
Oncoproteins/suppressors
Oncoproteins
Other
Anti-ANP32C antibody [EPR13354-70R] 图像
![Western blot - Anti-ANP32C [EPR13354-70R] antibody (ab189921)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1899211WB.jpg)
Western blot - Anti-ANP32C [EPR13354-70R] antibody (ab189921)
All lanes : Anti-ANP32C antibody [EPR13354-70R] (ab189921) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 27 kDa
Observed band size : 27 kDa
![Western blot - Anti-ANP32C [EPR13354-70R] antibody (ab189921)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab189921WBb.jpg)
Western blot - Anti-ANP32C [EPR13354-70R] antibody (ab189921)
All lanes : Anti-ANP32C antibody [EPR13354-70R] (ab189921) at 1/2000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 27 kDa
Observed band size : 27 kDa
![Immunoprecipitation - Anti-ANP32C [EPR13354-70R] antibody (ab189921)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1899211IP.jpg)
Immunoprecipitation - Anti-ANP32C [EPR13354-70R] antibody (ab189921)
Western blot analysis of ANP32C in MCF-7 cell lysate immunoprecipitated using ab189921 at 1/50 dilution (Lane1). Lane 2: PBS instead of MCF-7 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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