详细说明
概述
产品名称Anti-Elav-type RNA-binding protein ETR3抗体[EPR13374-2]
描述
兔单克隆抗体[EPR13374-2] to Elav-type RNA-binding protein ETR3
经测试应用WB,IHC-P,ICC/IF,Flow Cyt,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human Elav-type RNA-binding protein ETR3 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: O95319Run BLAST with
Run BLAST with 
阳性对照
Human fetal liver, K562, HeLa, MCF7, C6 and NIH 3T3 lysates; Human brain tissue; rat lung tissue; MCF7 and HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13374-2
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Chromatin Binding Proteins
DNA / RNA binding
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] 图像
![Western blot - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1864300WB.jpg)
Western blot - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 54 kDa
Observed band size : 54 kDa
![Western blot - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab186430WBb.jpg)
Western blot - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
All lanes : Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 54 kDa
Observed band size : 54 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab186430IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab186430HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1864300IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab186430IFb.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
![Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1864300FC.jpg)
Flow Cytometry - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/270 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
![Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1864300IP.jpg)
Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 [EPR13374-2] antibody (ab186430)
Western blot analysis of Elav-type RNA-binding protein ETR3 in HeLa cell lysate immunoprecipitated with ab186430 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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