概述

• 产品名称Anti-GATA3 (phospho S308)抗体[EPR18118]
     

• 描述

  兔单克隆抗体[EPR18118] to GATA3 (phospho S308)

• 特异性The immunogen sequence for ab186371 is identical to the sequence around S340 of GATA2 (UniProt P23769); however the phosphorylation of GATA2 on S340 has not been reported.

• 经测试应用WB,IHC-P,Dot Blot,IP

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GATA3 aa 300-400 (phospho S308).
  Database link: P23771

• 阳性对照    

• WB: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours. IHC-P: Human placenta tissue and Human transitional cell carcinoma tissues. IP: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR18118

• 同种型IgG

• 研究领域

• Developmental Biology

• Lineage specification

• Ectoderm

• Stem Cells

• Lineage Markers

• Ectoderm

• Epigenetics and Nuclear Signaling

• Transcription

• Domain Families

• Zinc Finger

• Immunology

• Adaptive Immunity

• T Cells

• Non-CD

Anti-GATA3 (phospho S308) antibody [EPR18118] 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)          

     

  Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)          

     

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

   

• 

  Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)          

     All lanes : Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371) at 1/10000 dilution
  
  Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
  Lane 2 : Jurkat whole cell lysates treated with 1mM 8-Bromo-cAMP for 6 hours
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 48 kDa
  Observed band size : 48 kDa
  
  
  Exposure time : 15 seconds    

  Blocking/dilution buffer: 5% NFDM/TBST.

   

• 

  Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)          

     

  GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/1500 dilution.

  Lane 1: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 µg (Input).

  Lane 2: ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

  Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 10 seconds.

   

• 

  Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (ab186371)          

     

  Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

  Blocking/Dilution buffer: 5% NFDM/TBST..

  Exposure time: 3 minutes.