详细说明
概述
产品名称Anti-GFP抗体[EPR14104-89]
描述
兔单克隆抗体[EPR14104-89] to GFP
经测试应用ICC/IF,IHC-P,WB
免疫原
Full length protein corresponding to Aequorea victoria GFP aa 1 to the C-terminus.
Database link: P42212Run BLAST with
Run BLAST with 
阳性对照
GFP transfected 293 cell lysate; GFP transgenic mouse colon tissue; GFP transgenic mouse liver tissue; GFP transfected 293 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR14104-89
同种型IgG
研究领域
Tags & Cell Markers
Fusion / Marker Proteins
GFP
Anti-GFP antibody [EPR14104-89] 图像
![Western blot - Anti-GFP [EPR14104-89] antibody (ab183735)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1837355WB.jpg)
Western blot - Anti-GFP [EPR14104-89] antibody (ab183735)
All lanes : Anti-GFP antibody [EPR14104-89] (ab183735) at 1/10000 dilution
Lane 1 : GFP transfected 293 cell lysate
Lane 2 : Non-transfected 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 27 kDa
Observed band size : 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP [EPR14104-89] antibody (ab183735)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183735IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP [EPR14104-89] antibody (ab183735)
Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse colon tissue (left) and normal mouse colon tissue (right) labeling GFP with ab183735 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP [EPR14104-89] antibody (ab183735)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab183735HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP [EPR14104-89] antibody (ab183735)
Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse liver tissue (left) and normal mouse liver tissue (right) labeling GFP with ab183735 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-GFP [EPR14104-89] antibody (ab183735)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1837355IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GFP [EPR14104-89] antibody (ab183735)
Immunofluorescent analysis of 4% paraformaldehyde-fixed GFP transfected 293 cells labeling GFP with ab183735 at 1/500 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).
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