详细说明
概述
产品名称Anti-CD10抗体[EPR5904-110]
描述
兔单克隆抗体[EPR5904-110] to CD10
经测试应用WB,IP,IHC-P
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human CD10 aa 50-500. The exact sequence is proprietary.
Database link: P08473Run BLAST with
Run BLAST with 
阳性对照
WB: LNCaP, Raji and Ramos whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and breast cancer tissues. IP: LNCaP whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR5904-110
同种型IgG
研究领域
Cancer
Tumor immunology
CD markers
Stem Cells
Mesenchymal Stem Cells
Surface Molecules
Stem Cells
Hematopoietic Progenitors
Hematopoietic Stem Cells
Human Lineage Negative
Immunology
Cell Type Markers
CD
Non-lineage
Immunology
Adaptive Immunity
B Cells
CD
Anti-CD10 antibody [EPR5904-110] 图像
![Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab208778WBa.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
All lanes : Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 85 kDa
Observed band size : 90-110 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab208778WBb.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size : 85 kDa
Observed band size : 90-110 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab208778HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab208778HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
![Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2087788IP.jpg)
Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)
CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.
Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.
VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: LNCaP whole cell lysate, 10µg (Input).
Lane 2: ab208778 IP in LNCaP whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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