详细说明
概述
产品名称Anti-DMAP1抗体[EPR18179]
描述
兔单克隆抗体[EPR18179] to DMAP1
经测试应用ICC/IF,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DMAP1 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NPF5阳性对照
WB: HeLa, Jurkat, 293, MCF7, Ramos, Neuro-2a, Raw264.7, PC12, NIH 3T3 cell lysates; HeLa nuclear fraction lysate. ICC/IF: HeLa and F9 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR18179
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Chromatin Modifying Enzymes
Acetylation
HAT
Epigenetics and Nuclear Signaling
DNA methylation
DNMTs
Anti-DMAP1 antibody [EPR18179] 图像
![Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188407407.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188407-F9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DMAP1 antibody [EPR18179] (ab188407)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic carcinoma cell line) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on F9 cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.![Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188407O2A.jpg)
Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
![Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1884073T3.jpg)
Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilution
Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 3 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
![Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188407ela.jpg)
Western blot - Anti-DMAP1 antibody [EPR18179] (ab188407)
All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
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