详细说明
概述
产品名称Anti-Caspase-9抗体[EPR18108]
描述
兔单克隆抗体[EPR18108] to Caspase-9
经测试应用WB,IP
种属反应性
与反应: Mouse, Human
免疫原
Recombinant fragment within Human Caspase-9 aa 100-350. The exact sequence is proprietary.
Database link: P55211Run BLAST with
Run BLAST with 
阳性对照
WB: HeLa whole cell lysates untreated and treated with staurosporine 1uM for 4 hours; NIH/3T3 whole cell lysates untreated and treated with staurosporine1uM for 4 hours; Human fetal brain, fetal heart, fetal kidney and fetal liver and pancreas lysates; Mouse heart and kidney lysates. IP: HeLa whole cell lysate treated with staurosporine 1uM for 4 hours.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR18108
同种型IgG
研究领域
Cancer
Cell Death
Apoptosis
Metabolism
Cancer
Cell Death
Apoptosis
Apoptotic Markers
Cytochrome C
Cancer
Cell Death
Apoptosis
Apoptotic Markers
Caspases
Metabolism
Pathways and Processes
Metabolism processes
Apoptosis
Cancer
Invasion/microenvironment
Apoptosis
Cytochrome C
Cancer
Invasion/microenvironment
Apoptosis
Caspases
Cell Biology
Apoptosis
Intracellular
Caspases etc
Caspases
Anti-Caspase-9 antibody [EPR18108] 图像
![Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185719OWB.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)
Predicted band size : 46 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase-9 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab185719 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab185719 was shown to specifically react with Caspase-9 when Caspase-9 knockout samples were used. Wild-type and Caspase-9 knockout samples were subjected to SDS-PAGE. ab185719 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.![Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185719WBa.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)
All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/10000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa whole cell lysate treated with staurosporine1uM for 4 hours
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 46 kDa
Observed band size : 35,37,46 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185719WBb.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)
All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/2500 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with staurosporine 1uM for 4 hours
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 46 kDa
Observed band size : 37,39,49 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185719WBc.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)
All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185719WBd.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)
All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185719WBe.jpg)
Western blot - Anti-Caspase-9 antibody [EPR18108] (ab185719)
All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 46 kDa
Observed band size : 37,39,49 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunoprecipitation - Anti-Caspase-9 antibody [EPR18108] (ab185719)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1857199IP.jpg)
Immunoprecipitation - Anti-Caspase-9 antibody [EPR18108] (ab185719)
Caspase-9 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with staurosporine 1uM for 4 hours with ab185719 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab185719 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate treated with staurosporine 10 µg (Input).
Lane 2: ab185719 IP in HeLa whole cell lysate treated with staurosporine.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185719 in HeLa whole cell lysate treated with staurosporine.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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